基于线粒体AMPK/SIRT1/PGC-1α通路研究玛咖改善大鼠中枢疲劳的作用机制
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1.山东省疾病预防控制中心;2.山东农业大学食品科学与工程学院 山东 济南

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山东省中医药科技项目;山东省医药卫生科技发展计划项目


To Study the Anti-central-fatigue Effect of Maca via Mitochondrial Biogenesis by AMPK/SIRT1/PGC-1 Pathway in Rat
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1.山东省疾病预防控制中心;2.Shandong Center for Disease Control and Provention;3.Shandong Agricultural University

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Shandong Province Traditional Chinese Medicine Science &Technology Development Plan;Shandong Province medical and health Science &Technology Development Plan

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    摘要:

    目的:探讨玛咖对中枢疲劳大鼠的改善作用及其可能机制。方法:40只SD大 鼠按体重随机分为空白对照组、模型对照组和玛咖低、中、高剂量(0.6、1.2、2.4g/kg.bw)组,除空白对照组外,其余各组采用冷水游泳、剥夺睡眠、束缚、夹尾等多种方式反复刺激大鼠制造中枢疲劳大鼠模型。连续灌胃35天后,对大鼠进行Morris 水迷宫、糖水偏爱度、悬尾实验等行为学测试和大鼠海马中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)和过氧化氢酶(catalase,CAT)等氧化应激指标检测,并以免疫印迹法检测大鼠海马AMPK/SIRT1/PGC1-α通路相关蛋白表达水平。结果:与模型对照组比较,玛咖能够减少中枢疲劳大鼠逃逸潜伏期、增加站台穿越次数、增加平台所在象限活动时间;升高糖水偏爱度,减少大鼠悬尾挣扎时间;SOD、CAT活性升高、MDA含量降低;AMPK/SIRT1/PGC1-α通路相关蛋白表达水平升高。结论:玛咖可以改善中枢疲劳大鼠的行为学指标和氧化应激指标,其机制可能与调节AMPK/SIRT1/PGC1-α通路相关蛋白有关。

    Abstract:

    Abstract:objective To examine the anti-central-fatigue function of maca and its underlying mechanism . Methods: A central fatigue rat model was established by multi-factor modeling method with cold-water swimming、sleep deprivation、restraining and tail-clamping. 0.6、1.2、2.4g/kg.bw maca were respectively treated to rat by gavage for 35 days. Behavioral testing was studied by morris water maze test, sucrose preference test and tail suspension test. The markers of oxidative stress in hippocampus such as superoxide dismutase (SOD) 、malondialdehyde (MDA) and catalase (CAT) was detected by test kits. The proteins connected with AMPK/SIRT1/PGC1-α signaling pathway in hippocampus were detected by western blot. Results: Compared with modle group,maca significantly decreased the escape latency and quadrant activity time and the tail suspension time and the content of MDA while increased the times of crossing the platform and the sucrose preference and the activities of SOD and CAT. And maca significantly upregulated the expression of the proteins connected with AMPK/SIRT1/PGC1-α signaling pathway in hippocampus. Conclusion: Maca can improve the indicators in central fatigue rats connected with behavioral testing and oxidative stress . The underlying mechanism may be related to its regulatory effect on the the proteins in AMPK/SIRT1/PGC1-α signaling pathway .

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  • 收稿日期:2025-02-19
  • 最后修改日期:2025-05-30
  • 录用日期:2025-06-05
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