Experimental animal anatomy plays a significant role in experimental zoology, veterinary medicine, and other life sciences. Integrating mind mapping into the entire teaching process can effectively aid teachers in optimizing the organization of teaching contents and stimulate students’ enthusiasm and self-motivation for learning. This manuscript aims to explore the feasibility, advantages, and challenges of applying mind mapping tools in experimental animal anatomy teaching. It analyzes different application scenarios from the perspectives of both teachers and students, aiming to provide practical teaching tools and methodologies for enhancing instructional effectiveness in experimental animal anatomy education.