Objective:The 4D-FastDIA proteomic method was employed to explore how Liqitongfu massage works in treating functional dyspepsia (FD), focusing on the differences in proteins.Methods:30rats were categorized into three groups: blank, model, and massage, with each group containing 10 rats, determined by a random number table approach. Treatment for both the model and massage groups included tail clips, ice water, and a non-standard diet. After successful modeling, the massage group was treated with qi-tongfu manipulation. Following a 14-day treatment period, an assessment was conducted on the overall health and digestive movement of rats in every group, along with monitoring the pathological alterations in the rats" stomach tissues and identifying the protein levels in each.Results:General conditions: The blank group had good mental state and was active, while the model group had poor mental state, poor activity, and decreased gastric empelling rate and small intestinal propulsion rate. The massage group had improved mental state, activity rate, gastric empelling rate and small intestinal propulsion rate. HE staining results: No clear infiltration of inflammatory cells was observed in the gastric mucosa, nor was there a notable variance in gastric histopathology across the three groups. Results of the proteomic analysis: In contrast to the standard group, the model group exhibited alterations in 263 proteins, while the massage group showed changes in 237 proteins when compared to the model group. There were differences between the model group and the normal group, but the massage could reverse the difference trend of 95 proteins, of which 16 were up-regulated and 79 were down-regulated.Conclusion:Qitongfu massage can effectively improve FD, which may be related to the regulation of related target proteins.