The Experimental Animal Center serves as the most crucial support platform for the development of disciplines such as science, technology, agriculture, medicine, research and teaching in universities, providing a venue for animal experiments for faculty and students. The facilities and equipment of the Experimental Animal Center are the most important safeguards for ensuring the quality of animal housing environments and the health of personnel. This paper summarizes the actual operation and maintenance of facilities and equipment and the management of troubleshooting in Yunnan University Experimental Animal Center in recent three years. This paper deeply analyzes practical experience in daily maintenance, troubleshooting, personnel training, safety management, and operational effectiveness evaluation. The aim is to provide a comprehensive reference for enhancing the stability, reliability, efficiency, and energy conservation of equipment operation in university experimental animal centers, ensuring the safe and efficient operation of these centers.