Objective To analyze the influence of Acta Laboratorium Animalis Scientia Sinica and Chinese Journal of Comparative Medicine through CNKI big data, objectively analyze and evaluate the data, display the current development status of the journals, and propose high-quality development suggestions. Method CNKI was used as the statistical data source, and relevant quantitative data from the "Annual Report on the Impact Factors of Chinese Academic Journals" were used. Bibliometric methods were employed to quantitatively analyze the publication characteristics indicators of the two journals from 2021 to 2023, including citation count, number of funded papers, publication delay, composite total citation frequency, and composite impact factors. Result During the statistical period of 2021-2023, the publication scale of the two journals was moderate, the number of funded papers was relatively high, the publication delay was relatively long, and there was significant room for improvement in the composite total citation frequency and composite impact factor. Conclusion The high-quality development of academic journals should focus on content construction, strengthen scientific research cooperation, do a good job in brand promotion, apply advanced technology, carry out professional writing guidance and training, and regularly conduct academic influence evaluations.