To strengthen the supervision of laboratory animal welfare and ethics, and ensure the animal welfare, a scientific regulatory system for the entire process was established. The system included 12 regulatory indexes. Targeted evaluation criteria were formulated based on the characteristics of the indexes. The methods of inspecting the sites and using specialized equipments such as small animal Micro CT and MRI were applied to supervise regulatory indexes. The system established a dynamic regulatory linkage mechanism that combines project approval review, process supervision, and final review. And the system implemented a regulatory process that combines online and offline methods. The system prevented the occurrence of incidents that violate technological ethics, standardized regulatory procedures, and provided reference for the supervision of welfare and ethics in the production and use of laboratory animal.