【Abstract】 Objective：To explore the mechanism by which "three techniques and three acupoints" massage improves the motor function of SNI rats by delaying denervated muscle atrophy. Methods: Thirty-six male SD rats were randomly divided into normal group (n=9), sham operation group (n=9), model group (n=9), and massage group (n=9). The model group and massage group were subjected to SNI model establishment by clamping method; the sham operation group only exposed the sciatic nerve without clamping. From the 7th day after surgery, the massage group rats were intervened. An intelligent massage technique simulation instrument was used to apply point, pull, and knead techniques to Yinmen, Chengshan, and Yanglingquan acupoints in sequence. Each acupoint was treated with each technique for 1 minute, once a day. After 10 interventions, a 1-day rest was taken, followed by another 10-day intervention; the sham operation group and model group rats only received the same duration of grasping and fixation treatment; the normal group maintained routine feeding conditions. The hind limb grip force was measured before modeling, after 10 and 20 interventions to evaluate muscle strength. After 20 massage sessions, the rats were sacrificed and the right gastrocnemius muscle was taken for HE staining to observe the muscle atrophy and measure the cross-sectional area of muscle fibers; RT-PCR was used to detect the expression of CCNB1, CNTF, and MEF2A mRNA in the right gastrocnemius muscle tissue; Western-blot was used to detect the expression of PAX3, Desmin, HGF, and MEF2C proteins in the right gastrocnemius muscle tissue; immunofluorescence was used to detect the expression of MEF2C protein in the affected gastrocnemius muscle. Results： Compared with the normal group and sham operation group, the hind limb grip force of the model group rats was significantly reduced (P<0.01); the muscle fibers of the model group rats were disordered, the muscle cells were significantly atrophied, and the cross-sectional area was significantly reduced (P<0.01), and the expression of CCNB1, CNTF, MEF2A mRNA and PAX3, Desmin, HGF, and MEF2C proteins was lower than that of the normal group and sham operation group (P<0.05). Compared with the model group, the hind limb grip force of the massage group rats was significantly increased (P<0.01), the muscle fibers were atrophied but arranged orderly without obvious disorder, and the cross-sectional area was significantly increased (P<0.01), and the expression of CCNB1, CNTF, MEF2A mRNA and PAX3, Desmin, HGF, and MEF2C proteins was significantly upregulated (P<0.05). Conclusions "Three techniques and three acupoints" massage can promote the expression of CCNB1, CNTF, MEF2A mRNA and PAX3, Desmin, HGF, and MEF2C proteins, promote the proliferation and differentiation of muscle satellite cells and repair nerve injury, delay denervated muscle atrophy, and improve the motor function of SNI rats.