Laboratory animal science has been established as the fundamental course in many disciplines such as clinical medicine and animal science, and artificial intelligence (AI) has been widely applied in clinical medicine and laboratory animal teaching. Here we summarize the application of AI based virtual simulation technology to assist experimental animal teaching, optimize experimental animal monitoring and analysis, and construct virtual animal models in teaching. Also, we list the domestic and international platforms as well as AI tools, and collect the advantages of AI to improve teaching efficiency. It is expected that the application of AI will not only break through the bottleneck of traditional teaching, but also will promote the integration and optimization of multiple disciplines in laboratory animal science, cultivate students’ creative thinking, and improve teaching quality.