OBJECTIVE: To compare the application value of rabbit brain fluid (TF) and lipopolysaccharide (LPS)-induced disseminated intravascular coagulation (DIC) model in medical laboratory teaching. METHODS: The two classical models were systematically compared from four dimensions: pathogenic mechanism, modeling technology, detection system, and teaching application, and the research results and teaching practice were integrated. Results: The rabbit brain fluid model simulates acute traumatic DIC through exogenous coagulation activation, and the LPS model replicates chronic infectious DIC through endogenous inflammation-coagulation cascade, which complement each other in terms of pathogenicity, index characteristics, and experimental skills training. CONCLUSION: The optimization strategy of mechanism-oriented-skill stratification-clinical mapping can significantly improve students" knowledge and practical ability in the complex pathological process of DIC, and provide a three-dimensional solution for medical laboratory teaching.