Objective: To evaluate the effects of "Tiao Ren Tong DU"(TRTD) electroacupuncture on hippocampal neural repair in post-stroke depression(PSD) rats, focusing on the regulation of hippocampal autophagy homeostasis via the Notch1/Hes1 signaling axis, and to compare its efficacy with fluoxetine.Methods :Adult male SD rats were subjected to middle cerebral artery occlusion (MCAO) followed by 21 days of chronic unpredictable mild stress(CUMS) to establish the PSD model. Successfully modeled rats were randomly divided into five groups: sham, MCAO, PSD, fluoxetine, and TRTD acupuncture groups. The treatment groups received either TRTD electroacupuncture or fluoxetine by gavage for 14 days. Brain injury and neurological recovery were assessed via TTC staining and modified neurological severity score(mNSS). Depressive-like behaviors were evaluated using the open field test(OFT) and sucrose preference test(SPT). Immunofluorescence staining was used to observe GFAP-positive signals in the hippocampal CA1 region. Western blot was performed to measure the protein levels of LC3-II, Beclin-1, GFAP, and NeuN in the hippocampus. The mRNA expression levels of Notch1 and Hes1 were detected by qPCR.Results: Behavioral tests showed that both fluoxetine and TRTD treatment significantly ameliorated neurological deficits and depressive-like behaviors in PSD rats compared to the model group(P<0.05). The TRTD group demonstrated superior improvement to the fluoxetine group in the open field test. At the molecular level, qPCR analysis revealed that both treatments significantly downregulated the mRNA expression of Notch1 and Hes1 in the hippocampus(P<0.05), indicating inhibition of the Notch1/Hes1 signaling axis. Accordingly, the model-induced activation of astrocytes was suppressed, as shown by decreased GFAP protein levels and a significant reduction in GFAP-positive cells in the hippocampal CA1 region(P<0.01). Concurrently, the treatments restored autophagy homeostasis, evidenced by significantly reduced LC3-II protein levels(P<0.05) and a decreasing trend in Beclin-1. These beneficial molecular and cellular changes ultimately promoted neuronal survival, as reflected by the recovery of NeuN expression.Conclusion: TRTD electroacupuncture may exert antidepressant and neuroprotective effects by inhibiting the overactivation of the Notch1/Hes1 signaling axis, thereby attenuating reactive astrogliosis and restoring hippocampal autophagy homeostasis. The multi-targeting regulation of TRTD may underlie its therapeutic advantages over fluoxetine.