The zebrafish, as a model organism, is widely used in life science research. However, its systematic integration into undergraduate experimental teaching systems requires further development. Based on the training objectives of cultivating “research orientation, systematic thinking, and innovation ability” in undergraduates, this study designed a comprehensive zebrafish experimental course focused on the functional evaluation of “anti-fatigue” and “anti-wrinkle” effects. The course transformed industry consortium standards (T/ZHCA014-2022) and established scientific research models into comprehensive experimental projects suitable for undergraduate education. By guiding students through the entire process of experimental design, model establishment, data collection and analysis, and academic report writing, it effectively fostered their scientific thinking and practical skills. Teaching practice has demonstrated that this experimental teaching model not only significantly enhanced students’ experimental operational skills, data analysis capabilities, and teamwork spirit but also achieved the teaching goals of integrating research into teaching and linking theory with practice. This model provides a replicable and scalable practical example for implementing innovative experimental teaching based on advanced model organisms at the undergraduate level, representing a valuable exploration for undergraduate teaching quality improvement projects.