| 葛文平,张旭,高翔,刘宁,张海涛,张晓娟.我国商业化SPF级小鼠病原体污染分析[J].中国比较医学杂志,2012,(3):65~68. |
| 我国商业化SPF级小鼠病原体污染分析 |
| Analysis of Microbial Contamination on Mainly SPF Mice Strains in China |
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| DOI：10.3969/j.issn.1671.7856.2012.003.014 |
| 中文关键词: 无特殊病原 小鼠 病原体检测 |
| 英文关键词:Specific pathogen free Mice strains Microbial monitoring |
| 基金项目:十一五新药专项支持(2008ZX09305-001)。［通讯作者］ 张晓娟,E-mail:zhangmice@hotmail.com。 |
| 作者 | 单位 | E-mail | | 葛文平 | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 | | | 张旭 | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 | | | 高翔 | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 | | | 刘宁 | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 | | | 张海涛 | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 | | | 张晓娟 | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 | zhangmice@hotmail.com |
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| Author Name | Affiliation | E-mail | | GE Wen-ping | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health
Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences
Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine
Peking Union Medicine College, Beijing 100021, China | | | ZHANG Xu | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health
Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences
Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine
Peking Union Medicine College, Beijing 100021, China | | | GAO Xiang | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health
Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences
Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine
Peking Union Medicine College, Beijing 100021, China | | | LIU Ning | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health
Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences
Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine
Peking Union Medicine College, Beijing 100021, China | | | ZHANG Hai-tao | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health
Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences
Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine
Peking Union Medicine College, Beijing 100021, China | | | ZHANG Xiao-juan | Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health
Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences
Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine
Peking Union Medicine College, Beijing 100021, China | zhangmice@hotmail.com |
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| 中文摘要: |
| 目的按现行国标《实验动物微生物学检测方法》和《实验动物寄生虫学检测方法》对我国商品化的无特殊病原体(specific pathogen free,SPF)小鼠进行微生物状况检测,为生产高质量的实验动物提供依据。方法对五家主要实验动物生产单位生产的ICR、KM、C57BL/6、BALB/c及BALB/c-nu 品系的SPF级小鼠进行随机抽检。检测抽检小鼠所携带的微生物和寄生虫情况。结果在抽检的SPF级小鼠中,病毒污染主要包括呼肠孤病毒Ⅲ型(Reo-3)、小鼠肺炎病毒(PVM)和多瘤病毒(POLY)； 主要细菌污染为肺炎克雷伯杆菌、金黄色葡萄球菌和绿脓杆菌。这些微生物病原体中,除条件性致病菌绿脓杆菌外,其他病原体对小鼠本身和实验研究均具有一定的影响。 |
| 英文摘要: |
| ObjectiveTo provide reference for producing a good laboratory animal production and management, we randomly analyzed microbes and parasites of Specific pathogen free(SPF)mice commonly used in China.Methods The current national standards《Laboratory animal—microbiological standards and monitoring》and《Laboratory animal—parasitic standards and monitoring》were used to tested the microbes and parasites in the samples of SPF mice strains commenly used in China, including ICR,KM,C57BL,BALB/c and BALB/c-nu.ResultsThe results showed that the main virus carried by SPF mice were Reo-3, PVM and POLY, the bacteria contamination included klebsiella pneumoniae, Staphylococcus aureus and Peudomonas aeruginosa. They all affect the mice and experiment in certain degree except for Peudomonas aeruginosa. |
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