ObjectiveDiscussion the common problem of tumor-burdened in node-mice,In order to improve the success rate of tumor burden. MethodThrough analysis the influence of various factors of tumor-burden in node-mice, seek the solution of problem in tumor burdended. ResultSummarized the various factors in the tumor burden, and put forward reasonable suggestions.ConclusionNode-mice tumor burden is the base of the oncology, drug and biological products of safety evaluation and effectiveness screening. This paper is to provide reasonable suggestions for the preparation of good nude mice tumor model.