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周俏苗.miR-124-3P 靶向调控 MAPK 14 对子痫前期大鼠胎盘滋养层细胞增殖及侵袭的影响[J].中国比较医学杂志,2020,30(6):1~9.
miR-124-3P 靶向调控 MAPK 14 对子痫前期大鼠胎盘滋养层细胞增殖及侵袭的影响
miR-124-3P affects proliferation and migration in preeclampsia pathogenesis by targeting MAPK 14
投稿时间:2020-01-08  
DOI:10. 3969 / j.issn.1671-7856. 2020. 06. 001
中文关键词:  miR-124-3P  MAPK 14  子痫前期  滋养层细胞  增殖  侵袭
英文关键词:miR-124-3P  MAPK 14  preeclampsia  trophoblasts  proliferation  invasion
基金项目:
作者单位E-mail
周俏苗 海南省妇女儿童医学中心,海口 570000 doctorxiao003@ 163.com 
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中文摘要:
      目的 探讨 miR-124-3P 通过靶向调控 MAPK 14,从而对子痫前期大鼠胎盘滋养层细胞增殖与侵袭产生作用。 方法 提取正常大鼠及模型大鼠胎盘滋养层组织及原代细胞,分组转染。 MTT、流式细胞术、Transwell 检测检测细胞活力、凋亡、周期、迁移及侵袭能力,qRT-PCR 及 Western blot 检测因子的表达水平。 验证 miR-124-3P< /i> 和 MAPK 14 的靶向关系。 结果 过表达 miR-124-3P 后,细胞增殖、侵袭能力降低,细胞阻滞在 G1 / G0 期,细胞凋亡增加;抑制 miR-124-3P 表达后,结果相反。双荧光素酶报告实验显示 miR-124-3P 与 MAPK 14 存在靶向关系。 抑制 MAPK 14 表达后,与过表达 miR-124-3P结果一致,细胞活力及侵袭能力降低,凋亡增加;过表达 MAPK 14 实验组与抑制 miR-124-3P表达结果一致。 结论 miR-124-3P< /i> 可靶向负调控 MAPK 14,对子痫前期大鼠胎盘滋养层细胞的增殖与侵袭产生影响。
英文摘要:
      Objective To explore the effects of miR-124-3P on the proliferation and invasion of placental trophoblasts during preeclampsia by targeting MAPK 14. Methods Preeclampsia rat models were established, followed by extraction of placental trophoblast tissue and primary cells from normal rats and model rats. Primary cells were manipulated by transfections. The MTT assay was used to determine cell viability; flow cytometry was used to detect apoptosis and cell cycle arrest; Transwell was used to determine the cell migration and invasion ability, and qRT-PCR and western blotting were used to determine the expression of miR-124-3P and MAPK 14 as well as the expression level of migration-related genes. Verify the binding relationship between miR-124-3P and MAPK 14. Results Compared with the control cells, miR- 124-3p overexpression reduced cell proliferation and invasion. Cells arrested at the G1 / G0 phase, and the apoptosis rate increased. Suppressing miR-124-3P expression produced opposite result . The dual luciferase reporter experiment showed that MAPK 14 was the target of miR-124-3P. Suppressing the expression of MAPK 14 produced result that were consistent with the miR-124-3Poverexpression effects, i.e., the cell viability and invasion ability decreased, and the apoptosis rate increased. In contrast, MAPK 14 overexpression in the experimental group was consistent with the effects of inhibition of miR-124-3P expression. Conclusions miR-124-3P negatively regulates MAPK 14 and affects the proliferation and invasion of placental trophoblasts in preeclampsia rats.
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