The mangiferin was investigated on the hepatoprotective effects in three kinds of models of experimentally-induced liver injuries in rats. The models were induced by acetaminophen, carbon tetrachloride, and D-GalN. The results showed that mangiferin caused significant reduction of the elevated enzyme levels of serum glutamate oxaloacetate transminase, serum glutamate pyruvate transminase in model rats, and alleviated the pathological damages in livers of the model rats. These findings are suggestive of the potent hepatoprotective effects of mangiferin.