Objective To explore a new immunological genetic therapy for glioma with the liposome-mediated transgenic method.Methods We introduced the pLXSN-expressed recombinated plasmid into the glioma cell line C6 of Wistar rat,then we pick up the positive clone by G418 screening,finally,got the expression of B7 gene in C6 cell line with PCR,RNA-slot hybridization.Results With PCR,we obtained 0.8Kb's segment of B7 gene from the genome of the B7-transdued C6 cell (C6pLXSN-B7); with RNA-slot hybridization using B7 as the probe,we found the transcription of B7 gene in the C6pLXSN-B7 cells;with anti-B7 the expression of B7 molecule on the surface of C6pLXSN-B7 cells,on the contrary,there was no expression of B7 in the glioma cells of the control group (C6pLXSN,C6).Conclusion The liposome-mediated B7 transgenic method can lead to the entire integration of B7 gene into the genome of C6 cells and the resulting stable and effective expression of B7 gene.