Objective Using the methods of RT-PCR and virus isolation for verifying the SARS infection animal model and evaluating the effect of vaccine. Method We chose 3 years old SPF macaques, and inoculated the SARS coronavirus by dropping in their nose. Those infected animals served as SARS model to test SARS candidate vaccine. Throat swab specimens and the plasma specimens were collected in different time after monkeys were infected. Result After challenged, the throat swab specimens from the SARS infection animal model are positive every day till death and the throat swab specimens from vaccine immunized monkeys are positive only at the first day by the method of RT-PCR .The plasma specimens from the monkeys of SARS infection animal model are positive and the plasma specimens from the vaccine immunized monkeys are negative by the method of RT-PCR.We isolate the SARS virus on the the throat swab specimens from the SARS infection animal model. Conclusion These methods are fast and efficient. They can be used to identify the SARS infection animal model , evaluate the efficiency of vaccine and screen medicines.