Objective To find a sort of cells which is more sensitive for the cytotoxicity experiment on bedding materials.Methods The acetone extractions of beddings with different concentration were added in mouse fibroblast,S 180_S-2D-9 cells and H 22_H-2D-8 cells.The total protein of cells was measured by the method of Comassie brilliant blue after being cultured 72 hours and the livability of cells was counted.Then, the sensitivity of the three cells was compared.Results The H 22_H-2D-8 cells were more sensitive in the range of 1-40 mg/ml.Conclusion H 22_H-2D-8 cells can be used in the cytotoxicity experiment on bedding materials.