Objective To investigate the expression of GLAST and GLT-1 in rats with epilepsy.Methods Glutamate transporter GLAST and GLT-1 genes expression were detected by real-time fluorescent quantitative PCR assay with TaqMan probe in different time after epileptic seizure.Results The expression amount of GLAST and GLT-1 in cerebrum were obviously lower than that of the control in different time after epileptic seizure(P < 0.01).Furthermore,the amount of expression had evidently difference in cerebrum in different time after epileptic seizure(P < 0.01).The expression amount of GLAST in cerebellum was not significantly different in different time after epileptic seizure(P> 0.05),but they were obviously lower than that in the control(P<0.01).However,GLT-1 was not expressed in cerebellum.Conclusion The expression amount decrease of GLAST and GLT-1 may be one of the factors of epileptic seizure.