Objective In order to evaluate the safety of use of per acetic acid(PAA)and sodium dichloroisocyanurate (ACL-60)toxicity. Method To Ccompare the results of acute and subacute toxicity test. Result The results of acute oral LD50 showed that PAA and ACL-60 are of low toxicity grade. PAA and ACL-60 disinfectants could cause moderate irritation to animal skin and eye, and 2 g/L peracetic acid was non-irritative to animal skin and slightly irritative to the eye. 112 mg/L ACL-60 could cause moderate irritation to Guinea pig skin. The rates of PCE micronuclear cells induced by 3 dosage were not significantly different from that of negative control group (P>0.05). Conclusion peracetic acid and sodium dichloroisocyanurate are mildly toxic and can be considered as safe disinfectant commonly used at low concentration.