Objective To establish the transgenic mouse of 24-dehydrocholesterol reductase gene(Dhcr24) to study the effect of Dhcr24 on metabolism in vivo.Method\ The mouse Dhcr24 was clone by RT-PCR and the sequence of the cDNA was confirmed by sequence analysis.The transgenic plasmid was constructed by inserting the Dhcr24 gene in the downstream of CMV promoter.The transgenic mice were generated by microinjection method and the genotype was detected by PCR.The expression levels of the gene were determined with RT-PC...