Objective To establish lentivirus-mediated in vitro gene delivery systems for porcine embryonic fibroblasts(PEFs) of Tibetan miniature pigs.Methods According to the standard protocol from Invitrogen,lentiviruses carrying EGFP gene were produced,followed by confirming that lentiviruses harboring EGFP gene were successfully made and PEFs were successfully infected through EGFP assay under fluorescent stereo microscope after infecting PEFs.Results PEFs of Tibetan miniature pigs were successfully and efficientl...