HE Lin1,2,WANG Lu-zeng1,ZHENG Zhi-hong1(1.The Key Lab of Transgenic Laboratory Animals of Liaoning Province,Department of Laboratory Animal Science,2.The Central Laboratory of the Fourth Hospital,China Medical University,Shenyang 110001,China)
R730.2
Objective To observe the changes of cell proliferation and migration abilities of the normal human embryonic lung fibroblasts(HELF) after stable transfection of Dicer gene.Methods The transfection of Dicer gene into HELF cells was done by liposome-mediated method.G418 was used to screen HELF cells stably expressing Dicer gene.PCR was used to detect the integration of Dicer gene to HELF cells.RT-PCR was used to detect the expression of mRNA,and Western blot was used to determine the Dicer protein expression ...
