Effect of Stable Transfection of Dicer Gene on the Function of Human Embryonic Luag Fibroblasts
Author:
Clc Number:

R730.2

  • Article
  • | |
  • Metrics
  • | | | | |
  • Comments
    Abstract:

    Objective To observe the changes of cell proliferation and migration abilities of the normal human embryonic lung fibroblasts(HELF) after stable transfection of Dicer gene.Methods The transfection of Dicer gene into HELF cells was done by liposome-mediated method.G418 was used to screen HELF cells stably expressing Dicer gene.PCR was used to detect the integration of Dicer gene to HELF cells.RT-PCR was used to detect the expression of mRNA,and Western blot was used to determine the Dicer protein expression ...

    Key words:Dicer;HELF
    Reference
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation
Share
Article Metrics
  • Abstract:1667
  • PDF: 0
  • HTML: 0
  • Cited by: 0
History
Article QR Code