Objective To establish model of continuous infusion of enteral nutrition and a method for obtaining lymph in rats. Method After rats received gastrostomy ，the silicone tube ，which freed to post back，was connected to the micro infusion pump through the swivel. After 5 days of continuous enteral nutrition the rats were opened the abdominal cavity and freed the intestinal lymph trunk，then the rats was subjected to intestinal ischemia for 60 minutes and reperfusion for 120 minutes，meanwhile，stealth inserted the silicon tube to collect lymph. Result After the first operation，the continuous infusion of enteral nutrition was smooth in rats and there were no death or complications symptoms. The lymph fluid was obtained by a simpler method，which success rate was higher than 90% in the second operation. Conclusion Model establishment of continuous infusion of enteral nutrition in rats could greatly facilitate to study the role of nutrients.The simple method to obtain the lymph provides greatly convenience for the study of lymph ingredients.