Objective To develop a novel device for chronic lumbar catheterization of the spinal subarachnoid space in rat with a perfect protection of the external portion of catheter，and more convenient for repeat injection. Methods Pathogen-free adult male Sprague-Dawley rats were used in this study. Rats were divided into two groups (n = 12 each) randomly. Rota-rod test and Tail-flick test were introduced to assess motor and sensory function respectively at one day preoperative and 1st，3rd，7th day postoperative. To evaluate functionality of the catheter，10 μL 2% lidocaine was injected in the catheter at 1st，3rd and 7th day post-catheterization. Results There were no mortality and overt paralysis in 2 groups. There is no significant difference in motor and sensory function between the two groups (P > 0. 05，T test). Most external portions were destroyed a few days post operation in control group，however in group with device，all the catheters worked well(P < 0. 05，χ2 test) and lidocaine test showed that functionality of the micro-catheters were normal 7 days after catheterization. Conclusion The novel device was reliable and convenient for repeat injection with a perfect protection function of the external portion of catheter，meanwhile it provided a more ideal model for chronic study in anesthesia，analgesia，neuroscience and relevant research.