Objective To determine the values of anatomical parameters of HBK-SPF ducks at age of 7 and 56 days old and compare the differences between the male and female ducks. Methods The values of anatomical parameters of 7- and 56-day-old HBK-SPF ducks were measured and the results were analyzed by t-test. Results There was a very significant difference between the left and right kidney values (P < 0. 01) ，and a significant difference of shank girth and cecum 2 values (P < 0. 05) between male and female ducks at the age of 7 days. At the age of 56 days，a very significant difference existed only of the thymus values (P < 0. 01) ，and a significant difference of the cecum 1 and rectum values (P< 0. 05) between the male and female ducks. Conclusion The values of some anatomical parameters are significantly different between male and female HBK-SPF ducks at age of 7 and 56 days.