Objective To investigate the distribution of Progesterone Receptor ( PR) in murine ovary，fallopian tube and uterus between the mice treated by estrus synchronization of PMSG ( pregnant mare serum gonadotropin) and of FSH ( follicle stimulating hormone) ． Methods Total 10 KM female mice were divided into two groups: PMSG and FSH treatment group，after the injection of these hormones for 48 h，all of their ovaries，oviducts and uterus were fixed by paraform，and stained by immunohistochemical staining to observe and analyze the distribution of PR in these tissues．Results The positively stained cell number in the primary and secondary ovarian follicles of the PMSG treatment group，were significantly higher than those of the FSH one(P ＜ 0. 05) ，the average absorbance in the ovarian follicles of the PMSG treatment group was significantly higher than that of the FSH one ( P ＜ 0. 05 ) ． In addition，The positively stained cell number in oviduct of the PMSG treatment group was significantly higher than those of the FSH group(P ＜ 0. 05) ，but the average absorbance is reversed． In endometrial epithelium and glandular epithelium，the positively stained cell number of the PMSG treatment group was significantly higher than that in the FSH one ( P ＜ 0. 05 ) ，the average absorbance in endometrial stroma and endometrium of the PMSG treatment group was significantly lower than that of the FSH one( P ＜0. 05) ． Conclusions PMSG and FSH may induce varying degrees of impact on the distribution of PR in murine ovaries，oviducts and uterus，the expression of PR of the PMSG treatment group was significantly higher than that of the FSH one．