Objective To study the genetic quality for six strains of inbred mice in Liaoning Province by microsatellites technology． Methods Ten microsatellites DNA locis and primers were selected in Mouse Genome Database and pertinent literature，and its polymorphism information content was rich，to analyzed on genetic polymorphism of mouse by PCR Amplification and PAGE electrophoresis． Results The same site results of PCR amplification showed polymorphism in different strains， and showed monomorphism in same strains， ten microsatellites DNA locis were homozygous state for all mouse． The genetic distance among C57BL /10 and C57BL /6J was 0． 1021，and it was 0. 1635 and 0. 1614 between BALB / c with C57BL /10 and C57BL /6J． Conclusions It was a feasible way to analyzed genetic monitoring among inbred mouse strains by ten microsatellites DNA locis．