Objective To investigate the potential infections of ten kinds of swine pathogens，including HCV，PRV，PRRSV，SIV，PPV，JEV，PCV2，Toxoplasma，chlamydial and Brucella in Tibet Minipigs． Methods Enzymelinked immunosorbent assay and serum agglutination test were used to detect antibodies of ten kinds of the swine diseases in blood samples，including ten samples from Tibet Minipigs sows of F0 generation which have been used swine fever vaccine， forty samples from 20-day-old suckling Tibet Minipigs and fifty samples from 3 ～ 5 months old Tibet Minipigs of F1 generation． Results The positive rate of HCV-Ab，PPV-Ab and JEV-Ab were 80. 0% 、80. 0% 、70. 0% in Tibet Minipigs sows，respectively; while 45. 0% 、25. 0% 、45. 0% in 20-day-old suckling Tibet Minipigs，respectively ;4. 0% 、76. 0% 、80. 0% in 3 ～ 5 months old Tibet Minipigs，respectively． The positive rate of PCV2-Ab is 60. 0% in Tibet Minipigs sows，5. 0% in 20-day-old suckling Tibet Minipigs，while 0% in 3 to 5 months old Tibet Minipigs． However，none of PRRSVAb，PRV-Ab，SIV-Ab，Toxo-Ab，chlamydial-Ab Brucella-Ab were detected in all Tibet Minipigs ． Conclusion In this study，PRRSV，PRV，SIV，Toxo，chlamydial and Brucella haven’t been found in Tibet Minipigs of different ages．