Objective To compare three methods for setting up artificial airway ( AA) of rabbit． Methods 30 New Zeland White rabbits were chosen and randomly divided into three groups: Group BOI ( group blind orotracheal intubation) ;Group COI ( group classic orotracheal intubation) ; Group TO ( group tracheotomy) ． The time for setting up artificial airway，the achievement ratio for the first time and the incidence of complication were observed． Results The shortest time for setting up artificial airway is Group BOI，showing a significant difference compared with other two groups (P ＜ 0. 05) ． The highest achievement ratio is Group TO，then Group BOI，and the lowest Group COI(P ＜ 0. 05) ． The highest incidence ratio of complication is Group TO，then Group COI，the lowest is Group BOI． Conclusions Blind intubation can be the first choice for setting up artificial airway for rabbit compared with classic oratracheal intubation and tracheotomy．