Objective To investigate haemostasis effect of thrombase retroperfusion through ureteral catheter on nephrorrhagia rats． Methods After renal biopsy with systemic heparinization，the models of nephrorrhagia rats with gross hematuria had been established，32 SD rats weighing 350 ～ 400g were divided into 4 groups randomly ( 8 rats in each group) ． All rats were retroperfused by three different doses of thrombase or NS through ureteral catheter respectively． Lab index: Urinary red cell，blood pigment，serum creatinine，bleeding time( BT) and clotting time( CT) were detected in all rats before retroperfusion，5 min and 40 min after retroperfusion． Results ①Compared with NS group，the level of red blood cell count in urine of the other 3 groups was significantly different respectively( P ＜ 0. 05) ，and the level of abovementioned index among high-dose group and middle-dose group was lower than low-dose group ( P ＜ 0. 05 ) ． While the difference of blood pigment between different distinctive dose groups and NS group was significant in 40 min after retroperfusion( P ＜ 0. 05) ． The level of above-mentioned index among high-dose group ( 500U /mL) and middling-dose group ( 250 U /mL) was distinguished higher versus low-dose group respectively ． ② Among 4 groups，the difference of bleeding time( BT) 、clotting time( CT) and serum creatinine between the former and the latter was not significant( P ＞ 0． O5 ) ．Conclusion All three different doses of thrombase had haemostasis effect on renal hemorrhage rats by retroperfusion through ureteral catheter，and the effects were related to the medicament and the dosage; while retroperfusion thrombase did not affect BT，CT and the level of serum creatinine of rats．