Objective To explore and establish the method for the skin photosensitization in guinea pigs．Methods TBS was used as the positive control，and the Acetone as the negtive control． The shaved skin of the guinea pigs was irradiated with UV-A radiation of 30 J/ cm2 repeated every 2 days for 3 consencutive days． After 4 weeks of initial sensitization，the skin of the guinea pigs was irradiated with UV-A radiation of 9 J/ cm2． Results Positive ratio of the negtive control 24h，48h and 72h after irridiating was 0，and the positive control was 100% ． Conclusions The skin photosensitization model was established successfully，and the TBS used as the positive control was available．