Objective To compare the differences of blood physiological and biochemical parameters among the Tibet minipig，Wuzhishan minipig and Bama minipig，bred in Guangzhou area． Methods Using automatic analyzer of blood cells and biochemistry to test 24 blood physiological and biochemical parameters． Results Among the 24 parameters，significant difference exist in Hgb，WBC，MONO% ，HCT% ，GLU and very significant difference exist in LYM% ，NEU% ，MCV，MCH，TP，ALB，BUN，and CREA． The Tibet minipig had the highest Hgb，HCT% ，MCV，MCH，TP，ALB，BUN，CREA． The Wuzhishan minipig had the highest WBC，LYM% ，and the Bama minipig had the highest NEU% ，MONO% ，and GLU． Conclusions The comparison of blood physiological and biochemical parameters among the three types of minipigs provide a theoretical basis for establishing the biological database of minipigs bred in Guangzhou area．