Objective To establish an simple and effective animal model of multiple organ dysfunction syndrome ( MODS) induced by lipopolysaccharide ( LPS) in sheep and observe the changes of blood gas，blood routine test and blood biochemistry． Methods Twelve sheep ( 6 males and 6 femals) were randomized into group 1 and group 2，six sheep in each group． After anesthesia，3 μg / kg LPS was infused intravenously within 30 minutes in the group 1 and 6 μg / kg LPS within 60 minutes in the group 2． The blood gas，blood routine test and blood biochemistry were measured at different time points． Results Two sheep in the group 1 ( 33. 3% ) and three sheep in the group 2 ( 50. 0% ) died after the intravenous injection of LPS． The oxygenation index was significantly decreased ( P ＜ 0. 05 or P ＜ 0. 01 ) ，aspartic transaminase ( AST) ，alanine aminotransferase ( ALT) ，total bilirubin ( TBIL) and creatinine ( CRE) were significantly increased ( P ＜0. 05 or P ＜ 0. 01 ) at different time points. The number of leukocytes was significantly decreased within 2 hours after intravenous injection of LPS，then markedly increased ( P ＜ 0. 05 or P ＜ 0. 01) ． Conclusions A sheep model of MODS has been successfully established by two doses of LPS． Significant changes of the indexes of blood gas，blood routine test and biochemistry can be seen in those model sheep．