Objective To establish a new method for rat kidney without traumatic local vascular drugs ，and after the treatment of its survival rate and traumatic carries on the observation． Methods Wistar rats，PE10 catheter through from the left common carotid artery- the descending aorta- the thoracic aorta- the abdominal aorta，arrived in renal artery directly to medicine． Statistical its survival rate and dosing renal arterial line pathology observation． Results Rats survival rate can reach 87. 5% ，and wont cause renal arterial thrombosis or vascular injury． Conclusions The method can be successfully for rats renal artery without traumatic local blood vessels to medicine，also can be extended for abdominal some of the other organs or the hind legs．