Objective Preparation and characterizing of porcine parvovirus ( PPV) hybridomas cells and their monoclonal antibody． Methods Two hybridomas cells were obtained by conventional methods． ． The hybridomas cells were analysed and identified by chromosome analysis，indirect ELISA，the immunoperoxidase monolayer assay ( IPMA) and indirect immunofluorescence ( IFA) methods． Results Two hybridomas cells were obtained and named 2H9 and 1F9 respectively． The chromosome numbers were between 90 to 110. Titration of the hybridomas culture supernatant were 1∶ 1× 104，and titration of ascitic fluid were 1 ∶ 1 × 107，their subclasses were IgG1，IgM，belonging to kappa chain，and without cross-reaction with PRRSV，CSFV，PRV，PCV-1，PCV2 and JEV． Furthermore，the specific reaction of antibodies and PPV in PK-15 cells was confirmed IPMA and IFA． Conclusion Two anti-PPV hybridomas cells were acquired and the characterizations of two monoclonal antibodies were confirmed ．