Experimental animals are an important tool and supporting condition for research，teaching，production，standardization． The species and strain of experimental animals used in research have been increased markedly alone with development at full speed of science and technique． But the biological diversity，ecological environment and human health have been threatened by experimental animals bio-safety issues in our country． This review analyzed the potential bio-safety hidden danger in experimental animals raising and use，investigated problems existed in experimentalanimals bio-safety control，and proposed some measures and advice to laboratory animals bio-safety．