bjective To observe the regulatory effect o f BIOSTIME probiotics on intestinal flora． Method Mice were divided into normal control group，BIOSTIME probiotics group． Control group fed with distilled water for 14 d．The BIOSTIME probiotics group fed with BIOSTIME probiotics for 14 d． The numbers of intestinal flora before and after experiments were detected． Result The numbers of intestinal Bifidobacterium of the groups fed with BIOSTIME probiotics superior to those of normal control group( P ＜ 0. 05) ，while the number of E． coli，Enterococcus ，Lactobacillus，Clostridium perfringens was not significantly changed( P ＞ 0. 05) ，compared with the data prior of feeding． Conclusion BIOSTIME has a regulatory effect on mouse intestinal flora．