Objective Chemotherapy-induced peripheral neuropathy ( CIPN) is the common adverse reactions of mutiple chemotherapy drugs． At present，CIPN model is most commonly established by injecting paclitaxel ( PTX ) intraperitoneally to SD rats． However，those methods differ a lot and the influence factors are still unknown． Thus in this paper we aim to find the influence factors and optimize an ideal model for CIPN research． Methods SD rats were injected i． p． every other day with PTX． Mechanical allodynia and mechanical hyperalgesia effects were examined by the behavioral test． Intraepidermal nerve fiber ( IENF) of the skin of hind foot was stained by immunofluorescence and morphology and number of mitochondrial on the sciatic nerve was detected with electron microscopy． Results Injection of 2 mg·kg － 1 PTX for 4 times had no effect on the weight of SD rats． No obvious mechanical abnormalities pain and mechanical allodynia was observed during the 34 d experimental duration in rats with the initial weight of 200 g． While in rats with the initial weight of 400 g，mechanical abnormalities pain was observed at the 17d，and mechanical hyperalgesia lasted from the 8 d to the 26 d． At the same time，density of IENF under the skin was significantly reduced after PTX treatment，and the proportion of abnormal mitochondria was also increased，showing severe peripheral neuropathy． Conclusions Rats with initial body weight of 400 g are more sensitive to establish PTX-induced peripheral neuropathy and are more suitable for CIPN research．