Objective To improve the consciousness of biosafety in livestock laboratory，and put forward the experience for reference of design，construction，application and management of livestock biosafety laboratory． Method It is investgated and analyzed that designing idea， equipment， facilities， animal experiments and livestock laboratory management in North American． Results The livestock biosafety laboratory is related to healthy progress of animal husbandry and social stabilization． The construction of livestock biosafety laboratory between home and aboard exists remarkably different． Conclusion We should use the experience of designed idea in building livestock biosafety laboratory of North American for reference，consummate the infrastructure of livestock biosafety laboratory and add corresponding equipment，and improve the biosafety consciousness of animal laboratory staff simultaneously．