ObjectiveTo determine whether the SPF hamsters transfered from building 204 to isolator workshop were microbially polluted using different trasport boxes, and select qualified boxes for laboratory animal transportation.MethodsUsing triptic soy agar (TSA) culture dishes, simulating laboratory animal transportation process, and compare the results of settling plates in different boxes. ResultsThe microorganisms in individually ventilated cages cages (IVC) could be controlled bellow 14 cfu/4 h, and the morphology was the same with the colony in SPF building, therefore, were accord with standard. But in the conventional transport boxes, the colony were above 70 cfu/4 h, which failed to meet the standards.Conclusions SPF hamsters are not microbially polluted during the transport from building 204 to the isolator workshop using IVC cages. IVC cages are much better than conventional trasport boxes and should be used in laboratory animal transportation. 