ObjectiveTo provide reference for producing a good laboratory animal production and management, we randomly analyzed microbes and parasites of Specific pathogen free(SPF)mice commonly used in China.Methods The current national standards《Laboratory animal—microbiological standards and monitoring》and《Laboratory animal—parasitic standards and monitoring》were used to tested the microbes and parasites in the samples of SPF mice strains commenly used in China, including ICR,KM,C57BL,BALB/c and BALB/c-nu.ResultsThe results showed that the main virus carried by SPF mice were Reo-3, PVM and POLY, the bacteria contamination included klebsiella pneumoniae, Staphylococcus aureus and Peudomonas aeruginosa. They all affect the mice and experiment in certain degree except for Peudomonas aeruginosa.