ObjectiveTo know the laboratory animal''s microbial quality in Hunan Province, and to provide the important basis for scientific management of experimental animals, to ensure the quality of laboratory animals, the experimental results and the occupational health and safety of animal science workers.Methods laboratory animals’ microbiological test in accordance with GB 14922.2-2001、GB/T 14926-2001 and GB/T 14926.21-2008.Sample testing with the principle of simple random on the breeding colony in the relevant unit. ResultsSPF rat, SPF mice, Normal New Zealand rabbits, guinea pigs’ batch qualified rate was 73.68%, 77.78%, 60.61% and100% respectively.Conclusions It is showed that microbiological quality of laboratory animals from 2006 to 2010 in Hunan province had a downward trend on the earlier stage, and had been improved greatly. Measures should be taken to strengthen the management of laboratory animals and to eliminate microbial infections in experimental animals.