ZHAO Chang-cheng
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State administration of Traditional Chinese medicine, Beijing 100021, China GAO Xi-qiang
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State administration of Traditional Chinese medicine, Beijing 100021, China WU Fang-xin
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State administration of Traditional Chinese medicine, Beijing 100021, China ZHANG Wei-lun
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State administration of Traditional Chinese medicine, Beijing 100021, China XUE Jing
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State administration of Traditional Chinese medicine, Beijing 100021, China WEI Qiang
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State administration of Traditional Chinese medicine, Beijing 100021, China QIN Chuan
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health; Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Disease Animal Models, State administration of Traditional Chinese medicine, Beijing 100021, China Objective To investigate the effect of IL-21 on secretion of IFN-γ in CD8+ T cells of rhesus monkeys infected by SHIV. MethodsThe in vitro study was carried out on the culture of CD8+ T cells isolated from rhesus monkey models infected by SHIV. CD8+ T cells were incubated with various concentrations of IL-21 (1～15 ng/mL). Then IFN-γ concentrations in the supernatant were detected by ELISA, RT-PCR was performed to detect the expression of IFN-γ mRNA in the CD8+ T cells, and the percentage of IFN-γ-positive CD8+ T cells was assayed by flow cytometry. ResultsIL-21 (10 ng/mL) significantly increased IFN-γ level (P <0.05) in the supernatant of CD8+ T cell culture, compared with that in the control group. The expression of IFN-γ mRNA was significantly enhanced. IFN-γ which requires de novo synthesis following activation peaked at 4 h post stimulation. ConclusionIL-21 can increase the secretion of IFN-γ in vitro.
