YU Wen-hai
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaHUANG Fen
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China YANG Feng-mei
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaLU Shuai-yao
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaZHAO Yuan
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaWANG Jun-bin
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaCHEN Li-xiong
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaLI Yan-yan
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaHE Zhan-long
Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming 650118, ChinaObjective To analyze the genetic diversity and phylogenetic of Macaca mulatta in Yunnan Province, which provide important reference and background data for researches of macaca mulatta protection and reasonable application. MethodsNinety six blood samples of Macaca mulatta were collected for amplifying the complete sequences of mitochondrial DNA control region by polymerase chain reaction (PCR). The variation sites, parsimony informative sites, haplotypes, haplotype diversity, and nucleotide diversity were analyzed using Mega 4.0 and DNA SP software. The phylogenetic tree was constructed by neighbor-joining (NJ) and minimum-evolution(ME) methods.ResultsOne hundred and forty-nine variation sites and 46 haplotypes were found in the ninety-sixth Macaca mulatta, the haplotype diversity (Hd) was 0.968±0.007 and nucleotide diversity (Pi) was 0.020. The phylogenetic analysis based on mitochondrial control region showed that these Macaca mulatta lived in Yunnan was divided into two sister groups geographically. ConclusionsThe results of this study indicate a high variation and phylogenetic evolution relationship of Macaca mulatta in Yunnan province.
