DING Ming
Laboratory Animal Center of Academy of Military Medical Sciences, Beijing 100071, China;Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070,ChinaFAN Qiang
Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070,ChinaSHI Yan-sheng
Laboratory Animal Center of Academy of Military Medical Sciences, Beijing 100071, ChinaZHANG Xiao-fei
Laboratory Animal Center of Academy of Military Medical Sciences, Beijing 100071, ChinaZHANG Guang-zhou
Laboratory Animal Center of Academy of Military Medical Sciences, Beijing 100071, ChinaLI Chun
The 208th hospital of PLA, Changchun 130062, ChinaBAI Jiey-ying
Laboratory Animal Center of Academy of Military Medical Sciences, Beijing 100071, ChinaLIU Zheng-fei
Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070,ChinaObjectiveTo detect the function of I BHV-1 TK-/gE-/ EGFP+ gene-deleted mutant virus strain, find a new method to prevent infectious bovine rhinotracheitis (IBR), the charaction of TK-/gE-/ EGFP+ gene-deleted mutant virus strain was further studied. Methods Southern hybridization, western dot blot and Plaques test were employed in this investment. ResultsTK gene is deleted and the mutant cannot produce glycoprotein E when gE gene is deleted. The diameter of mutant strain much smaller than that of field strain, the mutant strain grows on MDBK cells and the virus titer can keep 106.0TCID50/0.1 mL for more than 40 hours, which indicated that the mutant strain inherit instantly. Conclusion The gene-deleted mutant virus strain was successful constructed in this study, and the mutant virus strain was stable. This will be useful for the further study of IBR.
