ObjectiveTo explore the effect of ginsenoside Rb₃ on chromosome aberration in Chinese hamster lung (CHL) cells. MethodsTo determine the IC₅₀ of ginsenoside Rb₃ in CHL cells, and to establish the range of doses according to the IC50 and induction of chromosome aberration. The results were judged according to the positive response standard. ResultsNegative response was found at 6 h and 24 h after the treatment with ginsenoside Rb₃ and at 6 h after the addition of S₉ mixture. ConclusionsUnder the conditions used in this study, ginsenoside Rb₃ does not induce chromosome aberration in CHL cells in vitro.