ObjectiveTo establish a sheep model of multiple organ dysfunction syndrome (MODS) at high altitudes induced by lipopolysaccharide (LPS) and observe the pathological characteristics of organs. MethodsSixteen 1-year old sheep (male∶female = 1∶1) were randomly divided into moderate altitude group (1500 m) and high altitude group (3200 m). The MODS models were generated by intravenous infusion of LPS at a dose of 6 μg/kg. After 24 hours, the sheep were anesthetized, and samples of the lung, liver, heart, spleen, kidney and intestine tissues were taken and fixed in 10% formalin for histopathological examination. ResultsThe lung, liver, spleen and intestinal tissues had significant inflammatory changes in both MODS groups, and especially, the pathology scores of the lung and liver at high altitude MODS group were significantly higher than those in the moderate altitude MODS group. ConclusionsPathological damages of MODS at high altitude are more aggravated than those at moderate altitude.