Objective To establish Rhesus monkey model of hyperlipidemia to provide reliable animal model for the study of human hyperlipidemia. MethodsDesigned high fat experimental feed formulations and detected its nutrients to feed Rhesus monkey to arouse hyperlipidemias. 2 mouths、4 mouths、6mouths later, we monitored the body weight, serum total cholesterol( TC ), triglyceride ( TG ), high-density lipoprotein cholesterol (HDL-C ), low density lipoprotein cholesterol (LDL-C),and analyzed the trend of each index above. ResultsWhen it was 2 moths and 4 months later,the feed intake and energy intake of model group were lower than control group(P<0.05), but the body weight of model group was not significantly different from that of the control group( P > 0.05 );When it was 6 moths, the feed intake and energy intake of model group was not significantly different from that of the control group, and the body weight of model group were significantly different from that of the control group(P<0.05);When it was 2 moths later,the indexes of TC,TG,HDL-C and LDL-C of the model group were not significantly different from that of the control group ( P > 0.05 ).When it was 4 moths later,the indexes of TC,TG, LDL-C of the model group were significantly different from that of the control group(P<0.05). When it was 6 moths later,the indexes of TC,LDL-C of the model group were significantly different from that of the control group(P<0.05),and much higher than the normal levels,but the indexes of TG,HDL-C were not significantly different from that of the control group ( P > 0.05 ).According to the monitoring, TC and LDL-C showed a increase trend, TG showed a gradual recovery after the first descending trend,and the HDL-C was not significantly changed.ConclusionRhesus monkeys of the model group has a feature of high cholesterol and high low-density lipoprotein cholesterol. The paper has initialy established Rhesus monkey model of experimental hyperlipidemia.