ObjectiveTo observe the changes of blood biochemistry and blood gas and electrolytes in sub-health model rats caused by different factors. Method60 SPF male Wistar rats were randomly divided into 5 groups (n = 12):multifactor group (MF), warm swimming group (WS), sleep deficiency group (SD), purely constraint group (PC) and control group (C). We used the way of single stimulation such as warm swimming, sleep deficiency or complex stimulation to establish the sub-health models. Six rats were sacrificed 5 days after modeling. The others were sacrificed 3 days after recovery breeding. The serum ALT, AST, TP, ALB, BUN, CREA, GLU, TC, TG, CK, LDH levels and blood gas and electrolytes were detected. Result(1) Compared with the control group, the serum BUN and TG in the MF group were significantly lower (P<0.05, P<0.01) 5 days after modeling, while CREA, AST, CK, CK/AST and LDH levels were significantly increased (P<0.01). The levels of TC, CK, CK/AST and LDH in the WS group were also significantly increased (P<0.01) and GLU concentration in all the groups was significantly increased (P<0.05). After recovery breeding for 3 days, the levels of CREA, AST, CK, CK/AST and LDH in MF group were significantly higher than those in the control group (P<0.01), while the CK activity in the SD group was significantly higher than that in the control group (P<0.05). (2) Compared with the control group, PCO₂, Hct and Hb in the MF, SD and PC groups were significantly increased at 5 days after modeling (P<0.05, P<0.01), while Hct and Hb in the WS group were significantly increased and Na⁺ concentration decreased (P<0.01). PO₂ and SO₂% in the MF group were significantly decreased and K+ concentration was increased (P<0.05). The HCO-₃ concentration of all the groups was significantly increased. After recovery breeding for 3 days, except for pH and PCO₂ were significantly decreased in all model groups (P<0.05, P<0.01), PCO₂ in the MF, SD and PC groups was significantly increased and SO₂% was decreased (P<0.05, P<0.01), Na⁺ concentration in the MF and WS groups was still lower than that in the control group, and K⁺ and Mg⁺⁺ concentrations in the SD group were significantly higher than those in the control group (P<0.05, P<0.01). ConclusionsBlood biochemistry, blood gas and electrolytes indexes are differently changed in sub-health rats induced by different factors, thus these indexes could be used to evaluate the changes of blood microenvironment in the sub-health state. 